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A Picropolychrome Staining Technique Applied to Dupuytren’s Tissue

From the Plastic Surgery and Burns Research Unit, the Department of Biomedical Sciences and the Department of Pharmacology, University of Bradford, West Yorkshire, UK

Correspondence: Mr A. M. P. Fitzgerald, Plastic Surgery and Burns Research Unit, Department of Biomedical Sciences, University of Bradford, Bradford, West Yorkshire BD7 IDP, UK

Although the histology of Dupuytren’s tissue is well-documented, conventional stains do not distinguish between the different types of collagen which biochemistry and immunochemistry suggest are present. Dupuytren’s specimens [nodules (n = 26), cords (n = 15) and dermofasciectomies (n = 6)] were stained with haematoxylin and eosin, Van Gieson’s, Mallory’s, Masson’s, and Herovici’s picropolychrome stain, and examined for both cellularity and collagen staining characteristics. All stains illustrated the marked cellularity of the nodules, contrasting with a paucity of cells within the cords. The first four stains demonstrated uniformity of the collagen staining within the tissues. Herovici’s picropolychrome, however, showed distinct staining patterns for the dermis, nodules and cords, with both purple/red and blue areas. Other studies suggest that those fibres stained purple/red and blue are types I and III collagens respectively. These findings may shed further light on the tissue of origin of Dupuytren’s disease.

Journal of Hand Surgery (British and European Volume), Vol. 20, No. 4, 519-524 (1995)
DOI: 10.1016/S0266-7681(05)80167-6


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