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Gene Expression Analysis of Dupuytren’s Disease: The Role of TGF-β2

From the Division of Plastic Surgery, Stanford Hospital and Clinics, Stanford, CA and the Division of Plastic Surgery, Veteran’s Affairs Palo Alto Health Care System, Palo Alto, CA, USA

Correspondence: Andrew Y. Zhang, MD, Division of Plastic Surgery, 770 Welch Road, Fourth Floor, Stanford University Medical Center, Stanford, CA 94305, USA. Tel.: +1 650 813 9794; fax: +1 650 725 6605. E-mail: dr.zhang{at}gmail.com.

Dupuytren’s disease is characterised by nodular fibroblastic proliferation of the palmar fascia leading to contracture of the hand. Transforming growth factor beta (TGF-β) is thought to play a role in its pathogenesis. We performed a cDNA microarray analysis of Dupuytren’s diseased cord tissue with an emphasis on TGF-β isoforms. Normal-appearing transverse ligament of the palmar fascia from adjacent to the diseased cord and palmar fascia from patients undergoing carpal tunnel release were used as controls. TGF-β gene expression was confirmed by quantitative real-time polymerase chain reaction. Over 20 unique genes were found to be significantly up-regulated, including several previously reported genes. A dominant increase in TGF-β2 expression was seen in the cord tissue, whereas TGF-β1 and TGF-β3 were found not to be significantly up-regulated. Quantitative real-time polymerase chain reaction confirmed these findings. This gene expression profile allows for further experiments that may eventually lead to gene therapy to block the development and progression of Dupuytren’s disease clinically.

Key Words: microarray analysis • Dupuytren’s disease • TGF-β • gene expression

This version was published on December 1, 2008

Journal of Hand Surgery (European Volume), Vol. 33, No. 6, 783-790 (2008)
DOI: 10.1177/1753193408091352


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